Increased Production of Taxoids in Suspension Cultures of Taxus globosa after Elicitation

The objectives of this study were to investigate whether elicitors induce the production taxoids in Taxus globosa by testing hypothesis that cells a greater accumulation depending on type and concentration elicitor treatment tested. Cell cultures initiated from friable calli for producing using Gamborg medium supplemented with different initial combinations growth regulators as follows: naphthaleneacetic acid, benzylaminopurine, picloram, polyvinylpyrrolidone. cell suspension then used evaluating taxoid through treatments, such methyl jasmonate, ethanol, buthionine sulphoximine, hydrogen peroxide. showing best characteristics found 10.74 µM acid 3.33 picloram. highest biomass was obtained EtOH-2 (0.12 ± 0.005 mg·g−1 dry weight) after 8 days, while control at time 0.095 0.2 weight. exogenous application combination peroxide significantly increased 10-deacetylbaccatin (1662 µg·g−1 weight), cephalomannine (334.32 taxol (157.0 weight).